Each chapter is structured to provide detailed technical information interspersed with. In three sections of this book, leaders in the field, representing donor centers, hospital transfusion services, and reference laboratories in the us and overseas. Molecular immunohematology mih is a rapidly emerging. Transfusion in the age of molecular diagnostics hematology. Immunohaematology download ebook pdf, epub, tuebl, mobi. The book will be an approachable guide to learning about molecular testing as it relates to blood banking and immunohematology. Bag health care, lich, germany 40 and platforms beadchip rhce, version 1, and hea, version 1. Molecular immunohaematology has been shown to improve patients care to a level that cannot be achieved by any available serology alone 58. Intended use the hea beadchip kit is an in vitro diagnostic test intended for the molecular determination of allelic variants that predict erythrocyte antigen phenotypes in the rh c rh2, c rh4, e rh3, e rh5, v rh10, vs rh20. Rhcece733g produces one hrb phenotype and rhcece48c,733g makes another. Bioarrays beadchip assays deliver deep insight into antigen typing and provide the information needed to solve complex red blood cell and platelet compatibility cases. Rhd beadchip technology distributed by immucor for rhd genotyping. This beadchip includes the most valuable content from the humanimmuno v1 beadchip, supplemented with newly selected content produced by the immunology research community. It draws from analyses and case studies around the world and d.
Red blood cell antigen expression may be altered by variations in dna sequences. In regularly transfused patients, red cell genotyping was likely the most cost efficient and often the only way to accurately determine a patients rbc antigens. Technologist and international technologist in blood banking, bb ascp and bbascpi specialist and international specialist in blood banking, sbb ascp and sbbascpi suggested reading for examination preparation this list is intended only as a partial reference source. Beadchip molecular immunohematology toward routine donor and patient antigen profiling by dna analysis other authors. Jhang, md assistant director, transfusion medicine immunohematology demonstration of red cell antigenred cell antibody reactions is the key to immunohematology combination of antibody and antigen can result in observable reactions, most commonly. Molecular blood typing, grouping and infectious disease. Precisetype hea molecular beadchip test and are listed in the following table. Blood differs, however, from other biologicals in that it must be obtained from healthy individuals of the human race. John judd, fibms, mibiol emeritus professor university. A flexible array format for complex nucleic acid and protein analysis.
Two recently published papers have shown the feasibility of routinely applying molecular immunohematology techniques in hospital transfusion services. Blood differs, however, from other biologicals in that it must be obtained from healthy individuals of the human. Precisetype hea molecular beadchip test qualtex laboratories. The book covers the basics of red cell genetics and how molecular testing is used in the clinical setting. Precisetype tm hea test is the first and only fda approved test for extended red blood cell antigen typing immucors bioarray tm molecular beadchip tm assays are innovative tools in transfusion medicine that enhance donor and patient matching capability and complement our echo and neo automated serological testing systems. Europe molecular blood typing, grouping and infectious. We have entered the era of molecular immunohematology in which dna is analyzed to predict red blood cell and platelet phenotypes. Its distribution does not indicate endorsement by the american. Innovative molecular and immunohematology blood typing, grouping and infectious disease nat technologies, and their market applications table of contents 1. Molecular immunohematology at canadian blood services. The precisetype hea molecular beadchip test uses the proprietary elongation mediated multiplexed.
Analysis of molecular and immunohematology blood banking technologies, and strategic profiles of leading suppliers table of contents current and emerging technologies molecular diagnostics a. Precisetype hea molecular beadchip test 19020210 rev. Buy ebook beadchip molecular immunohematology by paul m. Toward routine donor and patient antigen profiling by dna analysis moulds, joann m. Hpa assay antigen coverage assay overview hpa1ahpa1b gpiiia hpa2ahpa2b gpib hpa3ahpa3b gpiib hpa4ahpa4b gpiiia hpa5ahpa5b gpia hpa6ahpa6b gpiiia hpa7ahpa7b gpiiia hpa8ahpa8b gpiiia hpa9ahpa9b gpiib. The bioarray rhce and rhd beadchips extensively identify rh. Although molecular testing will not be adopted by every laboratory right this moment, most laboratories will increasing utilize, and benefit from the advantages of, some degree of molecular testing in their blood bank. Thanks to increased knowledge of the molecular basis associated with many blood group antigens, it is currently possible to predict their.
Beadchip molecular immunohematology opens with a history of serology before it provides an overview of gene expression, how genetic variations translate into antigenic differences on the surfaces. This highthroughput molecular assay received fda pma approval in may, 2014 and is the only ivd approved molecular. Bioarray hpa beadchip is a highthroughput molecular assay that detects 22 human platelet antigens by multiplex dna analysis. Sandrine kapplergratias, thierry peyrard, pierreyves le pennec, jeanpierre cartron, philippe rouger, bachnga pham. Full text blood genotyping for improved outcomes in. Emerging molecular and immunohematology blood typing, grouping and infectious disease nat screening assays and companies developing new technologies and products table of contents 1. Genomic analyses of rh alleles to improve transfusion. Sloan 20101125 medical toward routine donor and patient antigen profiling by dna analysis. In three sections of this book, leaders in the field, representing donor centers. Immunohematology journal of blood group serology and molecular genetics volume 31, number 3, 2015 contents 103 original report first example of an fy01 allele associated with weakened expression of fya on red blood cells p. American proficiency institute 2011 1st test event can be costeffective and will likely become more so as the technology and the way to deliver the technology continues to improve.
Hea beadchip technology in immunohematology article pdf available in immunohematology american red cross 312. Bas117 pn 5002 rev a product line overview the bioarray product line provides a platform of solutions for molecular immunohematology. Toward routine donor and patient antigen profiling by dna analysis. Provide an explanation for why genetic methods can be used to predict blood group and platelet antigen status 2.
Clinical utility of red cell and platelet genotyping margaret a keller, phd national molecular laboratory american red cross margaret. This policy provides limitedcoverage for molecular phenotyping of erythrocyte antigens performed on the hea beadchip immucor, warren, nj, a single nucleotide polymorphisms snpbased microarray test. Lee beadchip molecular immunohematology toward routine donor and patient antigen profiling by dna analysis por disponible en rakuten kobo. Use of molecular testing in clinical laboratories requires knowledge of the molecular basis of blood group antigens and the availability of suitable genotyping methods that can be used in our testing environment. Immunohematology journal of blood group serology and molecular genetics volume 31, number 2, 2015 contents 62 molecular method an overview of the progenika id core xt. Subsequent chapters describe how molecular immunohematology is being used in various settings for different purposes. Table of contents 1 blood typing and grouping tests a. Bioarraytm hea beadchiptm molecular immunohematology mih is a rapidly emerging technology within transfusion medicine. Indeed, many laboratories and blood banks across the world have. It has specific effects on the human organism and dosage requirements, just as any other therapeutic substance.
Intended use the hea beadchip kit is an in vitro diagnostic test intended for the molecular determination of allelic. Infinium methylationepic beadchip kit 96 samples sign in to see pricing and favorite products. Beadchip molecular immunohematology opens with a history of serology before it provides an overview of gene expression, how genetic variations translate into antigenic differences on the surfaces of cells, and techniques that have been developed to detect genetic variations. Molecular blood typing, grouping and infectious disease nat. It is aimed at anyone interested in how mi is changing blood bank and transfusion medicine. Immucor precisetype human erythrocyte antigen hea molecular. Toward routine donor and patient antigen profiling by dna analysis springerverlag new york john j. The bioarray product line provides a platform of solutions for molecular immunohematology. Infinium immunoarray24 v2 kit autoimmune disorders content. Molecular biology center, blood transfusion service of hospital, s.
This book is for those who are learning about and working in the field of transfusion medicine and red cell genotyping, particularly those who are working or training as laboratory staff, supervisors, or physicians. An introduction to immunohematology download ebook pdf. Immunohematology for medical laboratory technicians is a text appropriate for all levels of laboratory science programs. Castilho 81 molecular method hea beadchip technology in immunohematology. Incorporating molecular typing in the routine of blood banks. Molecular immunohematology refers to the detection of the molecular genetic basis of. Please enter new favorite list name or select an existing list. Novel molecular techniques have been developed to identify the single nucleotide polymorphisms snps responsible for many of these variations. Jan 01, 2009 during the 20 th century, knowledge of blood groups grew from three antigens a, b, and o later shown to be h to nearly 300 discrete antigens. Antibody identification antigen typing of patients antigen typing of donors discrepancy resolution in both patients and donors screening for antigennegative donors. Methods in immunohematology download ebook pdf, epub.
It draws from analyses and case studies around the world and details many techniques used in many labs. Abo and rh typing key points can be done by tube, gel and solidphase assays two types of tests for rhd. The infinium immunoarray24 v2 beadchip is the nextgeneration genotyping array for detecting genetic variation in the human immune system. Analysis of polymorphisms emap technology to identify. Food and drug administration 10903 new hampshire avenue silver spring, md 20993 1888infofda 18884636332 contact fda. Most blood group antigens are accommodated in 30 blood group systems, 1, 3 and the gene or a small gene family for mns, rh, and chrg systems encoding each blood group system the gene encoding p1 remains to be published has been cloned and. The precisetype hea molecular beadchip test uses the proprietary elongationmediated multiplexed. Table of contents for 9781441975119 beadchip molecular. The third edition of the blood group antigen factsbook has been completely revised, updated and expanded to cover all 33 blood group systems.
Immunohematology and blood banking ii introduction blood for transfusion is a biologically active therapeutic substance. Wise, ms, mtascpsbb associate professor, georgia regents university american society for clinical laboratory science georgia 2015 annual meeting may 16, 2015 part i blood group systems. Pdf immunohematology download full pdf book download. Objectives molecular testing in the blood bank laboratory. Bas116 pn 5001 rev a product line overview the bioarray product line provides a platform of solutions for molecular immunohematology. Role of molecular techniques in transfusion medicine cont. Infinium methylationepic kit methylation profiling array. Humancytosnp ffpe12 beadchip for cytogenetic studies of. Chapter 3 describes the beadchip technology developed by bioarray solutions, the molecular immunohematology system that has been most widely adopted in blood bank laboratories. Immunohematology and blood banking i introduction blood for transfusion is a biologically active, therapeutic substance.
Beadchip molecular immunohematology toward routine donor. This 12sample beadchip array enables analysis of genetic and structural variation in the human genome, such as duplications, deletions, amplifications, copyneutral loh, and. Introduction to molecular immunohematology digital. Molecular immunohematology refers to the detection of the molecular genetic basis of an antigen, rather than the antigen alone. Genomic analyses of rh alleles to improve transfusion therapy. Journal of blood group serology and molecular genetics. Methods will be discussed, allele tables explained, and nuances taught, so that the reader will feel. Identify molecular basis of unusual serological results onestep, automated, objective antibody detection and identification transfected cells as immunogens conversion of igg mabs to igm determine origin of engrafted leukocytes in a stem cell recipient. It has specific effects on the human organism and dosage requirements just as any other therapeutic substance. Educational commentary molecular testing of blood antigens cont. Hea beadchip technology in immunohematology italian. It brings together research and clinical practice from wellknown experts in the field of blood group serology and molecular immunogenetics. It then describes in detail the beadchip technology developed by. Beadchip molecular immunohematology into the field of transfusion diagnostics, during which period antigen determinations for 200,000 donors and recipients have been completed.
Review of automated and semiautomated molecular and immunohematology analyzers. Innovative molecular and immunohematology blood typing. Analysis of current and emerging molecular blood typing, grouping and infectious disease nat screening assays. Red cell genotyping was performed using commercially available kits bagene rhtype version 2. Attempts made to make molecular matches for patients with anti hrb or antihrs hrb negative blood is acceptable but a more exact match can be made using molecular results ex. Blood genotyping for improved outcomes in chronic transfusion. For the beadchip, the combined price for the dna extraction and kit is.
534 1194 1520 873 914 1212 1550 1297 18 257 311 287 1534 259 1114 1515 238 1326 919 448 1441 420 298 343 624 421 544 73 730 1316 1085 1225 158 823 159 653 496 118 34 1433 100 1237 956 567 391